Knockdown of Mythimna separata chitinase genes via bacterial expression and oral delivery of RNAi effectors
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چکیده
منابع مشابه
Knockdown of Mythimna separata chitinase genes via bacterial expression and oral delivery of RNAi effectors
BACKGROUND RNAi (RNA interference) is a technology for silencing of target genes via sequence-specific manner. RNAi technology has been used for development of anti-pathogenic crops. In 2007, development of transgenic plants resistant to insect herbivore using RNAi technology was first reported, leading to a burst of efforts aimed at exploitation of RNAi mechanism and control strategy against v...
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RNA interference (RNAi) represents a high effective mechanism for specific inhibition of mRNA expression. Besides its potential as a powerful laboratory tool, the RNAi pathway appears to be promising for therapeutic utilization. For development of RNA interference (RNAi)-based therapies, delivery of RNAi-mediating agents to target cells is one of the major obstacles. A novel strategy to overcom...
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Sensory system plays important roles in a wide array of insect's behavior and physiological events, including the host landing and locating, feeding, flying, sex responding, mating and oviposition which happen independently and in sequence. The armyworm Mythimna separata (Lepidoptera: Noctuidae) of migratory insect is destructive for alimentarn crop and economic crop throughout the world. Here ...
متن کاملInsecticidal compounds from Tripterygium wilfordii active against Mythimna separata.
In the course of screening for novel naturally occurring insecticides from plants, the ethanol extract of the root bark of Tripterygium wilfordii Hook f. was found to show insecticidal activity against larvae of Mythimna separata Walker. Three active compounds were isolated by bioassay-guided fractionation of the extract and characterized as triptolide (1), triptonide (2) and euonine (3) by IR,...
متن کاملCloning, Expression and Purification of Subunit H of Vacuolar H+-ATPase from Mythimna separata Walker (Lepidoptera: Noctuidae)
The vacuolar (H+)-ATPase (V-ATPase) of insect, which is composed of membrane-bound V0 complex and peripheral V1 complex, participates in lots of important physiological process. Subunit H, as a subunit of V1 complex, plays a vital role in bridging the communication between V1 and V0 complexes and interaction with other proteins. Yeast subunit H has been successfully crystallized through express...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2017
ISSN: 1472-6750
DOI: 10.1186/s12896-017-0328-7